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z stack images  (Nikon)


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    Structured Review

    Nikon z stack images
    Z Stack Images, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59909 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/z+stack+images/Objectives/pmc12857274-201-0-10
    Average 99 stars, based on 59909 article reviews
    z stack images - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Hematopoietic EphA4 deficiency alters microglial heterogeneity and improves chronic spatial memory after brain injury
    Article Snippet: TUNEL staining was conducted using Click-iT Plus TUNEL Assay 647 (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. Z-stack images were captured using a Nikon ECLIPSE Ti2 inverted confocal microscope equipped with a motorized stage and a Nikon C2 laser system. .. Quantification of peripheral immune cells and resident microglia was conducted using the Optical Fractionator probe within MBF Biosciences’ Stereo Investigator software (version 2017.03).

    Article Title: Comprehensive analysis of extracellular matrix remodelling via cyclophilin inhibition in human models of alcohol-related liver fibrosis.
    Article Snippet: .. For ECM Fibrillar Feature Alignment using AFT (Alignment by Fourier Transform) workflow, two coverslips were stained per condition and 12 Z-stack images using a 60X objective lens were taken/ coverslip using a Nikon A1R Confocal Microscope (Nikon, Japan). .. Primary antibodies Epitope Company Host/isotype and clone Cat number Lot number Dilution RRID Antibodies for HSC staining α-SMA Invitrogen Mouse/IgG2a, clone 1A4 14–9760-82 2798495 1:500 AB_2,572,996 VIM R&D Rat/IgG2A clone 280,618 MAB2105 UUQO223061 1:500 AB_2241653 SOX9 Millipore Rabbit/IgG polyclonal AB5535 3450032 1:500 AB_2239761 Collagen I/II/III/IV/V Novus Rabbit/IgG polyclonal PA1–85324 H00218 1:200 AB_930067 Antibodies for CDM staining Collagen I/II/III/IV/V Novus Rabbit/IgG polyclonal PA1–85324 H00218 1:200 AB_930067 Collagen I Proteintech Mouse/IgG1 clone 1E9A7 67288–1-Ig 10020286 1:200 AB_2882554 Collagen III Proteintech Rabbit/IgG polyclonal 22734-1-AP 00086215 1:500 AB_2879158 Collagen IV Novus Goat/IgG polyclonal NBP1–26549 H2915-RM83 1:75 AB_1853202 FN Abcam Rabbit/IgG polyclonal AB23750 GR3386400– 1 1:500 AB_447655 TABLE 4 Secondary antibodies used for immunofluorescent staining.

    Article Title: Single‐Cell Morphomechanics of Prostate Cancer‐Associated Fibroblasts Identifies Distinct Features Associated with Patient Outcome
    Article Snippet: .. For comparison of nuclear volume with its projected area, Z‐stack images were acquired with a 40x objective and 0.6 μm step size on a Nikon Ti‐E confocal microscope with a spinning disk. ..

    Article Title: Hematopoietic EphA4 deficiency alters microglial heterogeneity and improves chronic spatial memory after brain injury.
    Article Snippet: TUNEL staining was 440 conducted using Click-iT Plus TUNEL Assay 647 (Thermo Fisher Scientific) according to 441 the manufacturer's protocol. .. Z-stack images were captured using a Nikon ECLIPSE Ti2 442 inverted confocal microscope equipped with a motorized stage and a Nikon C2 laser 443 system. ..

    Inverted Microscopy:

    Article Title: HIF-1α+ CD4 T cells coordinate a tissue resident immune cell network in the lung
    Article Snippet: Sections were mounted using SlowFade Diamond Antifade Mountant (Thermo Fisher) to preserve Egln3-YFP fluorescence. .. Z-stack images (10μM to 30μM, step size of 0.9μM) were acquired on a Nikon ECLIPSE Ti2 inverted microscope, equipped with a X-light V3 spinning disk confocal module and a 40X Apo Plan lambda, NA=0.95 objective. ..

    Article Title: HIF-1α + CD4 + T cells coordinate a tissue-resident immune cell network in the lung.
    Article Snippet: Sections were mounted using SlowFade Diamond Antifade Mountant (Thermo Fisher) to preserve Egln3-YFP fluorescence. .. Z-stack images (10μM to 30μM, step size of 0.9μM) were acquired on a Nikon ECLIPSE Ti2 inverted microscope, equipped with a X-light V3 spinning disk confocal module and a 40X Apo Plan lambda, NA=0.95 objective. ..

    Staining:

    Article Title: Comprehensive analysis of extracellular matrix remodelling via cyclophilin inhibition in human models of alcohol-related liver fibrosis.
    Article Snippet: .. For ECM Fibrillar Feature Alignment using AFT (Alignment by Fourier Transform) workflow, two coverslips were stained per condition and 12 Z-stack images using a 60X objective lens were taken/ coverslip using a Nikon A1R Confocal Microscope (Nikon, Japan). .. Primary antibodies Epitope Company Host/isotype and clone Cat number Lot number Dilution RRID Antibodies for HSC staining α-SMA Invitrogen Mouse/IgG2a, clone 1A4 14–9760-82 2798495 1:500 AB_2,572,996 VIM R&D Rat/IgG2A clone 280,618 MAB2105 UUQO223061 1:500 AB_2241653 SOX9 Millipore Rabbit/IgG polyclonal AB5535 3450032 1:500 AB_2239761 Collagen I/II/III/IV/V Novus Rabbit/IgG polyclonal PA1–85324 H00218 1:200 AB_930067 Antibodies for CDM staining Collagen I/II/III/IV/V Novus Rabbit/IgG polyclonal PA1–85324 H00218 1:200 AB_930067 Collagen I Proteintech Mouse/IgG1 clone 1E9A7 67288–1-Ig 10020286 1:200 AB_2882554 Collagen III Proteintech Rabbit/IgG polyclonal 22734-1-AP 00086215 1:500 AB_2879158 Collagen IV Novus Goat/IgG polyclonal NBP1–26549 H2915-RM83 1:75 AB_1853202 FN Abcam Rabbit/IgG polyclonal AB23750 GR3386400– 1 1:500 AB_447655 TABLE 4 Secondary antibodies used for immunofluorescent staining.

    Comparison:

    Article Title: Single‐Cell Morphomechanics of Prostate Cancer‐Associated Fibroblasts Identifies Distinct Features Associated with Patient Outcome
    Article Snippet: .. For comparison of nuclear volume with its projected area, Z‐stack images were acquired with a 40x objective and 0.6 μm step size on a Nikon Ti‐E confocal microscope with a spinning disk. ..

    Confocal:

    Article Title: Single‐Cell Morphomechanics of Prostate Cancer‐Associated Fibroblasts Identifies Distinct Features Associated with Patient Outcome
    Article Snippet: .. For comparison of nuclear volume with its projected area, Z‐stack images were acquired with a 40x objective and 0.6 μm step size on a Nikon Ti‐E confocal microscope with a spinning disk. ..

    Spinning Disk:

    Article Title: Single‐Cell Morphomechanics of Prostate Cancer‐Associated Fibroblasts Identifies Distinct Features Associated with Patient Outcome
    Article Snippet: .. For comparison of nuclear volume with its projected area, Z‐stack images were acquired with a 40x objective and 0.6 μm step size on a Nikon Ti‐E confocal microscope with a spinning disk. ..

    other:

    Article Title: Endothelin B receptor inhibition rescues aging-dependent neuronal regenerative decline
    Article Snippet: Sections were mounted and coverslipped using VECTASHIELD anti-fade mounting media with DAPI (Vector Laboratory, Cat# H-2000).



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